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| Registros recuperados: 68 | |
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| Benítez Alemán, Héctor Eduardo. |
| La identificación precisa de heces de carnívoros silvestres es importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento... |
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Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2291 |
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| Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse. |
| Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001 |
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| Matte,Eunice M.; Castilho,Camila S.; Miotto,Renata A.; Sana,Denis A.; Johnson,Warren E.; O'Brien,Stephen J.; Freitas,Thales R. O. de; Eizirik,Eduardo. |
| The puma is an iconic predator that ranges throughout the Americas, occupying diverse habitats. Previous phylogeographic analyses have revealed that it exhibits moderate levels of genetic structure across its range, with few of the classically recognized subspecies being supported as distinct demographic units. Moreover, most of the species' molecular diversity was found to be in South America. To further investigate the phylogeographic structure and demographic history of pumas we analyzed mtDNA sequences from 186 individuals sampled throughout their range, with emphasis on South America. Our objectives were to refine the phylogeographic assessment within South America and to investigate the demographic history of pumas using a coalescent approach. Our... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Mammals; Mitochondrial DNA; Molecular time estimate; Phylogeography. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400018 |
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| Azevedo,Marisa F.C.; Oliveira,Claudio; Pardo,Belén G.; Martínez,Paulino; Foresti,Fausto. |
| The fish order Pleuronectiformes, composed of 14 families, has two suborders: Psettodoidei (with one family) and Pleuronectoidei (with thirteen families). The relationships among families of Pleuronectoidei and among the genera of their families have extensively been debated and a consensus has not yet been reached. In the present study, partial sequences of the 12S and 16S mitochondrial rRNA genes were obtained from 19 species belonging to the families Achiridae, Bothidae, Cynoglossidae, Paralichthyidae, Pleuronectidae, Scophthalmidae, and Soleidae. Additional sequences of 42 pleuronectiform species were obtained from GenBank. Phylogenetic analyses were conducted by the methods of maximum-parsimony, maximum-likelihood and Bayesian inference. Our results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Flatfishes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200023 |
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| Hilsdorf,Silva; Oliveira,Claudio; Lima,Flávio César Thadeo de; Matsumoto,Cristianne Kayoko. |
| The genus Brycon, the largest subunit of the Bryconinae, has 42 valid species distributed from southern Mexico to the La Plata River in Argentina. Henochilus is a monotypic genus, comprising a single species (H. wheatlandii) found in the upper Rio Doce basin. In the present study, partial sequences of the mitochondrial gene 16S were obtained for fifteen species of Brycon and for Henochilus wheatlandii. The results showed that the genus Brycon is paraphyletic, since Henochilus is the sister-group of B. ferox and B. insignis. The most basal species analyzed were the trans-Andean species B. henni, B. petrosus, and B. chagrensis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phylogeny; Molecular systematics; Mitochondrial DNA; Fish evolution; Systematic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200034 |
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| Rodrigues,Rosa; Schneider,Horacio; Santos,Simôni; Vallinoto,Marcelo; Sain-Paul,Ulrich; Sampaio,Iracilda. |
| The acoupa weakfish (Cynoscion acoupa - Sciaenidae) is a marine species of croaker with estuarine-dependent behavior, found in the western Atlantic from Panama to Argentina. It is one of the most exploited food fish on the northern coast of Brazil. In this study, DNA sequences were determined from the entire control region (D-loop) of the mitochondrial genome of 297 individuals collected during seven different months between December 2003 and August 2005 on the northern coast of Brazil (Amapá and Pará). Genetic variability expressed by haplotype (h = 0,892) and nucleotide (pi = 0,003) diversities were low compared to other heavily exploited marine fish species from the western Atlantic and eastern Asia. AMOVA depicted a lack of genetic structuring among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cynoscion acoupa; Acoupa weakfish; Sciaenidae; Low genetic diversity; Mitochondrial DNA; D-loop. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300015 |
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| Sodré,Davidson; Rodrigues-Filho,Luis F.S.; Souza,Rosália F.C.; Rêgo,Péricles S.; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo. |
| Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcharhinus limbatus; Control region; Fishery stocks; Mitochondrial DNA; Population genetics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500008 |
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| Faria,Patrícia J.; Baus,Erika; Morgante,João S.; Bruford,Michael W.. |
| Little information is available about the population structure of communally nesting terns (Sternidae) and skimmers (Rynchopidae) throughout the world. In order to fill this gap, a survey of molecular markers was carried out for six species of terns (Anous stolidus, Sterna hirundinacea, S. fuscata, S. superciliaris, Thalasseus maximus and Phaetusa simplex) and one species of skimmer (Rynchops niger). First, we describe the results of the construction of genomic DNA libraries and document problems encountered during this procedure. Secondly, we tested the cross-amplification of 18 microsatellite loci previously described for related species (the number of polymorphic loci ranged from three to seven). Thirdly, we tested the usefulness of mtDNA (control... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; Microsatellites; Mitochondrial DNA; Terns. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400029 |
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| Tada,Susely F.S.; Souza,Anete P.. |
| The potato (Solanum tuberosum L.) mitochondrial cox3/sdh4/pseudo-cox2 gene cluster has previously been identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In our present study we used Southern blotting using sdh4 and cox2 probes to show that the sdh4 and cox2 genes are clustered in the mitochondria of potato, soybean and pea. Northern blotting revealed cotranscription of sdh4 and cox2 in potato but not in cauliflower, indicating that these genes are not clustered in cauliflower. A putative recombination point was detected downstream of the cox2 pseudogene (pseudo-cox2) in potato mitochondrial DNA (mtDNA). This sequence corresponds to a 32 bp sequence which appears to be well-conserved and is adjacent to the terminals of some... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Rearrangement; Plants; Solanum tuberosum; Cox2. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100017 |
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| Ianella,Patrícia; Albuquerque,Maria do Socorro Maués; Paiva,Samuel Rezende; Egito,Andréa Alves do; Almeida,Leonardo Daniel; Sereno,Fabiana T. P. S.; Carvalho,Luiz Felipe Ramos; Mariante,Arthur da Silva; McManus,Concepta Margaret. |
| Abstract The first horses were brought to Brazil by the colonizers after 1534. Over the centuries, these animals evolved and adapted to local environmental conditions usually unsuitable for exotic breeds, thereby originating locally adapted Brazilian breeds. The present work represents the first description of maternal genetic diversity in these horse breeds based on D-loop sequences. A D-Loop HSV-I fragment of 252 bp, from 141 horses belonging to ten Brazilian breeds / genetic groups (locally adapted and specialized breeds) were analysed. Thirty-five different haplotypes belonging to 18 haplogroups were identified with 33 polymorphic sites. Haplotype diversity (varying from 0.20 to 0.96) and nucleotide diversity (varying from 0.0039 to 0.0239) was lower... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Genetic characterization; Equus caballus; Locally adapted breeds; Animal genetic resources. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400604 |
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| Liu,Yongzhi; Ao,Liying; Ding,Haitao; Zhang,Dongli. |
| Abstract The aim was to study the frequencies of common deafness-related mutations and their contribution to hearing loss in different regions of Inner Mongolia. A total of 738 deaf children were recruited from five different ethnic groups of Inner Mongolia, including Han Chinese (n=486), Mongolian (n=216), Manchurian (n=24), Hui (n=6) and Daur (n=6). Nine common mutations in four genes (GJB2, SLC26A4, GJB3 and mitochondrial MT-RNR1 gene) were detected by allele-specific PCR and universal array. At least one mutated allele was detected in 282 patients. Pathogenic mutations were detected in 168 patients: 114 were homozygotes and 54 were compound heterozygotes. The 114 patients were carriers of only one mutated allele. The frequency of GJB2 variants in Han... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sensorineural hearing loss; GJB2; SLC26A4; GJB3; Mitochondrial DNA; Inner Mongolia. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400567 |
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| Xia,Peng; Radpour,Ramin; Zachariah,Rebecca; Fan,Alex Xiu Cheng; Kohler,Corina; Hahn,Sinuhe; Holzgreve,Wolfgang; Zhong,Xiao Yan. |
| Quantification of circulating nucleic acids in plasma and serum could be used as a non-invasive diagnostic tool for monitoring a wide variety of diseases and conditions. We describe here a rapid, simple and accurate multiplex real-time PCR method for direct synchronized analysis of circulating cell-free (ccf) mitochondrial (mtDNA) and nuclear (nDNA) DNA in plasma and serum samples. The method is based on one-step multiplex real-time PCR using a FAM-labeled MGB probe and primers to amplify the mtDNA sequence of the ATP 8 gene, and a VIC-labeled MGB probe and primers to amplify the nDNA sequence of the glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) gene, in plasma and serum samples simultaneously. The efficiencies of the multiplex assays were measured in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Circulating cell-free DNA; Mitochondrial DNA; Nuclear DNA; Real-time PCR; Quantitative PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100003 |
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| Registros recuperados: 68 | |
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